Journal: Regenerative Therapy

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats

doi: 10.1016/j.reth.2025.101049

Figure Lengend Snippet: Effects of Hypo-BMSCs-Exos on IL-1β-induced chondrocyte senescence. A: Immunofluorescence staining revealed changes in p16, p21, and p53 in IL-1β-induced chondrocytes following intervention with Norm-BMSCs-Exos and Hypo-BMSCs-Exos. B: Quantitative analysis of immunofluorescence staining. C: Western blot detection of p16, p21, and p53 expression in chondrocytes. D: Statistical analysis of gray values and relative values for corresponding protein bands. E: SA-β-gal activity in chondrocytes across different groups assessed using SA-β-gal staining kit, bar = 75 μm. F: Counting and statistical analysis of SA-β-gal positive cells in chondrocytes across groups. G: Flow cytometry analysis of mean fluorescence intensity for reactive oxygen species (ROS). H: Statistical analysis of ROS expression levels across groups. Results are presented as Mean ± SD. Intergroup statistical significance is indicated as: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 (n = 3).

Article Snippet: The cells were then washed three times with PBS, with each wash lasting 5 min. After blocking with bovine serum albumin (BSA) for 20 min at room temperature and subsequent washing with PBS, the cells were incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (Servicebio, China), Collagen II (Bisso, China), aggrecan (Bisso, China), ADAMTS-5 (Bisso, China), MMP-13 (Affinity, USA), p16 (Abcam, USA), p21 (Affinity, USA), and p53 (Proteintech, USA).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Activity Assay, Flow Cytometry, Fluorescence

Journal: Biomaterials Research

Article Title: Liposome-Encapsulated Rutin Attenuates Cisplatin-Induced Ototoxicity via Suppression of P53-Associated Oxidative Injury

doi: 10.34133/bmr.0324

Figure Lengend Snippet: Effects of Lip-Rutin on CDDP-induced OC-1 apoptosis and Western blot analyses. (A) Flow cytometry assay of Annexin V/PI to assess apoptosis in OC-1 cells across different treatment groups. (B) Quantification of apoptotic cells from flow cytometry data ( n = 3). (C to J) Representative Western blot images and relative expression of P53, CytC, cleaved caspase-3, caspase-9, Bcl-2, Bax, and Bcl-xl ( n = 3). *** P < 0.001 compared to the control group. ### P < 0.001 compared to the CDDP group.

Article Snippet: P53 and Bcl-2 antibodies were sourced from MedChemExpress (China).

Techniques: Western Blot, Flow Cytometry, Expressing, Control